蛋白酶体活性分析试剂盒说明书
产品名称：蛋白酶体活性分析试剂盒
英文名称：Proteasome Activity Assay Kit
货号：K245-100
分类：原装进口
规格：100T
英文简介：Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eukaryotes (and in some prokaryotes). They are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7 - 8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. BioVision’s Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
试剂盒中文简介:蛋白酶体活性分析试剂盒具有检测类糜蛋白酶活性的优势，利用AMC标记的底物自由释放的，高荧光强度的AMC来指示蛋白酶的活性.本试剂盒也包含阳性对照（具备显著蛋白酶体活性的Jurkat细胞裂解液）和一种特异的，可以抑制蛋白酶调控的所有蛋白水解活性的抑制剂MG-132，这些可以特异的检测样本中，可能存在具有蛋白水解作用的蛋白酶体的活性.
产品背景: 蛋白酶体是存在于所有真核生物（部分原核生物）的细胞核与细胞质内一类巨大蛋白复合物（20s，26s），它的功能是降解或者再循环被泛素标记的蛋白质.靶蛋白被降解成为7-8个氨基酸多肽已被随后进一步降解.20s装配体带有类糜蛋白酶，类胰蛋白酶功能蛋白酶结构和类半胱天冬酶活性区域.
产品特点: 1、简单的一步法操作，整个实验仅需30 - 60分钟；2、准确易用.
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