延胡索酸检测试剂盒说明书
产品名称：延胡索酸检测试剂盒
英文名称：Fumarate Assay Kit
货号：K633-100
品牌：原装进口
规格：100T
保存建议: 请在接收到延胡索酸检测试剂盒产品后，置于-20℃保存.
试剂盒简介: 延胡索酸检测试剂盒可以检测各种样品中延胡索酸的含量，操作简便，结果灵敏.试剂盒中的延胡索酸酶混合物能特异性识别延胡索酸并以之为底物，成比例地产生颜色反应.故延胡索酸的含量可以通过测定比色度（λ = 450 nm）来定量.检测极限低至1nmol延胡索酸/孔.
产品背景: 延胡索酸（Fumarate）是细胞通过Kreb循环消化食物产生ATP的中间产物.在哺乳动物肝脏细胞中，延胡索酸是尿素循环的产物之一，释放到胞质后转化成苹果酸，然后变成草酰乙酸，过程中产生NADH.人类皮肤被阳光晒后会自然地产生延胡索酸，这个物质能已经被用于治疗牛皮癣（一种可能是由于皮肤中延胡索酸生产途径被破坏导致的疾病）.延胡索酸还常被用于饮料、发酵粉和糖果制品的添加剂.
产品特点: 1、步骤简单，只需40分钟；2、操作快速并且方便
Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 μM).如与英文说明书有异，以英文说明书为准。
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